COPS3 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

COPS3 Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240918E3

Kat-Nr. : 83822-2-PBS

Synonyme

240918E3, COP 9 signalosome complex subunit 3, JAB1-containing signalosome subunit 3

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  WB analysis using 83822-2-RR (same clone as 83822-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83822-2-RR (COPS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83822-2-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83822-2-RR (same clone as 83822-2-PBS)

  WB result of COPS3 antibody (83822-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COPS3 transfected HeLa cells. This data was developed using the same antibody clone with 83822-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 83822-2-RR (same clone as 83822-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83822-2-RR (COPS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83822-2-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 83822-2-RR (same clone as 83822-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using COPS3 antibody (83822-2-RR, Clone: 240918E3 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83822-2-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 83822-2-RR (same clone as 83822-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using COPS3 antibody (83822-2-RR, Clone: 240918E3 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83822-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83822-2-RR (same clone as 83822-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COPS3 antibody (83822-2-RR, Clone: 240918E3 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83822-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00797-1

  Cytometric bead array standard curve of MP00797-1, COPS3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83822-4-PBS. Detection antibody: 83822-2-PBS. Standard: Ag32825. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83822-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83822-2-RR against Human COPS3 were performed. The affinity constant is 1.29 nM.

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Geprüfte Anwendungen

Produktinformation

83822-2-PBS bindet in WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA COPS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: COPS3 fusion protein Ag32825
• Vollständiger Name: COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis)
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC001891
• Gene symbol: COPS3
• Gene ID (NCBI): 8533
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COPS3 possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17.