COPS4 Polyklonaler Antikörper

COPS4 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 10464-1-AP

Synonyme

COPS, COPS 4, CSN4, JAB1 containing signalosome subunit 4, JAB1-containing signalosome subunit 4

---

• 

  WB analysis of mouse brain using 10464-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10464-1-AP (COPS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10464-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10464-1-AP (COPS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 10464-1-AP

  IP result of anti-COPS4 (IP:10464-1-AP, 4ug; Detection:10464-1-AP 1:300) with PC-3 cells lysate 1800ug.

• 

  IHC staining of human placenta using 10464-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10464-1-AP (COPS4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10464-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10464-1-AP (COPS4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, PC-3-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

10464-1-AP bindet in WB, IHC, IP, ELISA COPS4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COPS4 fusion protein Ag0735
• Vollständiger Name: COP9 constitutive photomorphogenic homolog subunit 4 (Arabidopsis)
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 40 kDa, 46 kDa
• GenBank-Zugangsnummer: BC004302
• Gene symbol: COPS4
• Gene ID (NCBI): 51138
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an ess ential regulator of the ubiquitin conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. CSN4 gene encodes a component of CSN complex, COP9 signalosome complex subunit 4 (COPS4). The ubiquitin-proteasome system plays a major role in the rhythmic accumulation and turnover of molecular clock components. A recent study in Drosophila indicates that CSN lie in a common pathway leading to light-dependent degradation of clock protein Timeless (TIM).

Publikationen

Species	Application	Title
		J Cell Sci; A nuclear export sequence promotes CRM1-dependent targeting of the nucleoporin Nup214 to the nuclear pore complex.; Authors - Mohamed Hamed
mouse	WB	Neuropsychiatr Dis Treat; COP9 Signalosome Subunit 3 Restricts Neuroinflammatory Responses During Cerebral Ischemia/Reperfusion Injury Through Stabilizing Suppressor of Cytokine Signaling 3 Protein.; Authors - En Liang
human	WB	bioRxiv; Crosslinking-mediated Interactome Analysis Identified PHD2-HIF1α Interaction Hotspots and the Role of PHD2 in Regulating Protein Neddylation; Authors - Haiping Ouyang

{{ptg:RelatedPrimaryAntibodies}}