COPS4 Polyklonaler Antikörper

COPS4 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10464-1-PBS

Synonyme

COPS, COPS 4, CSN4, JAB1 containing signalosome subunit 4, JAB1-containing signalosome subunit 4

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  WB analysis of mouse brain using 10464-1-AP (same clone as 10464-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10464-1-AP (COPS4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10464-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 10464-1-AP (same clone as 10464-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10464-1-AP (COPS4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10464-1-PBS in a different storage buffer formulation.

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  IP experiment of PC-3 using 10464-1-AP (same clone as 10464-1-PBS)

  IP result of anti-COPS4 (IP:10464-1-AP, 4ug; Detection:10464-1-AP 1:300) with PC-3 cells lysate 1800ug. This data was developed using the same antibody clone with 10464-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 10464-1-AP (same clone as 10464-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10464-1-AP (COPS4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10464-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 10464-1-AP (same clone as 10464-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10464-1-AP (COPS4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10464-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10464-1-PBS bindet in WB, IHC, IP, Indirect ELISA COPS4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COPS4 fusion protein Ag0735
• Vollständiger Name: COP9 constitutive photomorphogenic homolog subunit 4 (Arabidopsis)
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 40 kDa, 46 kDa
• GenBank-Zugangsnummer: BC004302
• Gene symbol: COPS4
• Gene ID (NCBI): 51138
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an ess ential regulator of the ubiquitin conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. CSN4 gene encodes a component of CSN complex, COP9 signalosome complex subunit 4 (COPS4). The ubiquitin-proteasome system plays a major role in the rhythmic accumulation and turnover of molecular clock components. A recent study in Drosophila indicates that CSN lie in a common pathway leading to light-dependent degradation of clock protein Timeless (TIM).

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