COPS6 Polyklonaler Antikörper

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COPS6 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10256-1-PBS

Synonyme

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  WB analysis using 10256-1-AP (same clone as 10256-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

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  WB analysis of 3T3-L1 using 10256-1-AP (same clone as 10256-1-PBS)

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10256-1-AP (COPS6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 10256-1-AP (same clone as 10256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10256-1-AP (COPS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 10256-1-AP (same clone as 10256-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10256-1-AP (COPS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

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  IF Staining of COLO 320 using 10256-1-AP (same clone as 10256-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10256-1-AP (same clone as 10256-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COPS6 antibody (10256-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10256-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10256-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA COPS6 und zeigt Reaktivität mit Affe, Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Affe, Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COPS6 fusion protein Ag0331
• Vollständiger Name: COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis)
• Berechnetes Molekulargewicht: 327 aa, 36 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC002520
• Gene symbol: COPS6
• Gene ID (NCBI): 10980
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The COPS6 protein, also named mov34, is a 34 kDa subunit of COP9 signalosome, a highly conserved eight-subunit protein complex that functions as an important regulator in multiple signaling pathways (PMID: 38308184). This protein belongs to translation initiation factor 3 (eIF3) superfamily. It is involved in the regulation of cell cycle and acts as the regulatory subunit of the 26S proteasome.

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