COPS8 Rekombinanter Antikörper

COPS8 Rekombinant Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240195B1

Kat-Nr. : 83312-1-PBS

Synonyme

COP9, 240195B1, COPS8/COP9, CSN8, hCOP9

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  WB analysis using 83312-1-RR (same clone as 83312-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83312-1-RR (COPS8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83312-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83312-1-RR (same clone as 83312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83312-1-RR (COPS8 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83312-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83312-1-RR (same clone as 83312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83312-1-RR (COPS8 antibody) at dilution of 1:250 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83312-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 83312-1-RR (same clone as 83312-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using COPS8 antibody (83312-1-RR, Clone: 240195B1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83312-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00336-2

  Cytometric bead array standard curve of MP00336-2, COPS8 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83312-2-PBS. Detection antibody: 83312-1-PBS. Standard: Ag0143. Range: 0.625-40 ng/mL.

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  Affinity and Kinetic Characterization of 83312-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83312-1-RR against Human COPS8 were performed. The affinity constant is 1.67 nM.

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Geprüfte Anwendungen

Produktinformation

83312-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA COPS8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: COPS8 fusion protein Ag0143
• Vollständiger Name: COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis)
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC003090
• Gene symbol: COPS8
• Gene ID (NCBI): 10920
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COPS8 is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases.