COPZ1 Polyklonaler Antikörper

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• KD/KO Validated

COPZ1 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 20440-1-AP

Synonyme

CGI 120, CGI-120, Coatomer subunit zeta 1, Coatomer subunit zeta-1, COPZ

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  WB analysis using 20440-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20440-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 20440-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20440-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20440-1-AP (COPZ1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 20440-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using COPZ1 antibody (20440-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COS-7-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below

Produktinformation

20440-1-AP bindet in WB, IF/ICC, ELISA COPZ1 und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COPZ1 fusion protein Ag14234
• Vollständiger Name: coatomer protein complex, subunit zeta 1
• Berechnetes Molekulargewicht: 177 aa, 20 kDa
• Beobachtetes Molekulargewicht: 17-20 kDa
• GenBank-Zugangsnummer: BC002849
• Gene symbol: COPZ1
• Gene ID (NCBI): 22818
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Blood; A new severe congenital neutropenia syndrome associated with autosomal recessive COPZ1 mutations; Authors - Natalia Borbaran Bravo
human	WB	MedComm (2020); Chemoproteomics enables identification of coatomer subunit zeta-1 targeted by a small molecule for enterovirus A71 inhibition; Authors - Xiaoyong Li
mouse	WB	Biochim Biophys Acta Gen Subj; COPZ1 regulates ferroptosis through NCOA4-mediated ferritinophagy in lung adenocarcinoma; Authors - Anbang Wu ; KD Validated
human	WB	Genes (Basel); A Comprehensive Pan-Cancer Analysis of the Regulation and Prognostic Effect of Coat Complex Subunit Zeta 1; Authors - Ye Hong ; KD Validated