COQ7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

COQ7 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(23)

Rezensionen (1)

Kat-Nr. : 15083-1-AP

Synonyme

CAT5, CLK1, EC:1.14.99.60, Timing protein clk-1 homolog, Ubiquinone biosynthesis monooxygenase COQ7

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  WB analysis of mouse liver using 15083-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15083-1-AP (COQ7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15083-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15083-1-AP (COQ7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15083-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15083-1-AP (COQ7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15083-1-AP

  IP result of anti-COQ7 (IP:15083-1-AP, 4ug; Detection:15083-1-AP 1:500) with mouse liver tissue lysate 6000ug.

• 

  IHC staining of human liver cancer using 15083-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15083-1-AP (COQ7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 15083-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 15083-1-AP (COQ7 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, Mausherzgewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 22 publications below
• IF: See 1 publications below

Produktinformation

15083-1-AP bindet in WB, IHC, IF, IP, ELISA COQ7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COQ7 fusion protein Ag7161
• Vollständiger Name: coenzyme Q7 homolog, ubiquinone (yeast)
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 20~24 kDa
• GenBank-Zugangsnummer: BC003185
• Gene symbol: COQ7
• Gene ID (NCBI): 10229
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COQ7(Ubiquinone biosynthesis protein COQ7 homolog) catalyzes the production of coenzyme Q (CoQ) in mitochondria. The predicted protein contains 179 amino acids, is mostly helical, and contains an alpha-helical membrane insertion. It has a potential N-glycosylation site, a phosphorylation site for protein kinase C and another for casein kinase II, and 3 N-myristoylation sites. This protein has 2 isoforms produced by alternative splicing with MW 20-24 kDa.

Publikationen

Species	Application	Title
human	WB,IF	Nat Cell Biol; A nuclear role for the respiratory enzyme CLK-1 in regulating mitochondrial stress responses and longevity.; Authors - Richard M Monaghan
human	WB	J Clin Invest; ADCK4 mutations promote steroid-resistant nephrotic syndrome through CoQ10 biosynthesis disruption.; Authors - Ashraf Shazia S ; KD Validated
mouse	WB	Nat Commun; Hepatic HuR modulates lipid homeostasis in response to high-fat diet.; Authors - Zhuojun Zhang
mouse	WB	EMBO Mol Med; β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.; Authors - Agustín Hidalgo-Gutiérrez
mouse	WB	Redox Biol; The Q-junction and the inflammatory response are critical pathological and therapeutic factors in CoQ deficiency.; Authors - Pilar González-García
mouse	WB	Acta Pharmacol Sin; Dysregulation of iron homeostasis and methamphetamine reward behaviors in Clk1-deficient mice.; Authors - Peng-Ju Yan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Ying (Verified Customer) (04-26-2021)

It's super clean and works great.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse and human fibroblasts, mouse tissues and human muscle biopsy samples