CORIN Rekombinanter Antikörper

CORIN Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240339A10

Kat-Nr. : 83566-1-RR

Synonyme

TMPRSS10, serine peptidase, CRN, 240339A10

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  WB analysis of rat heart using 83566-1-RR

  rat heart tissue were subjected to SDS PAGE followed by western blot with 83566-1-RR (CORIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 83566-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83566-1-RR (CORIN antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 83566-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 83566-1-RR (CORIN antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83566-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83566-1-RR against Human CORIN were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83566-1-RR bindet in WB, IHC, ELISA CORIN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CORIN fusion protein Ag15898
• Vollständiger Name: corin, serine peptidase
• Berechnetes Molekulargewicht: 1042 aa, 117 kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: BC110451
• Gene symbol: CORIN
• Gene ID (NCBI): 10699
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CORIN is a transmembrane type II serine protease expressed in cardiomyocytes that converts pro-atrial natriuretic peptide into atrial natriuretic peptide, a cardiac hormone that regulates blood pressure. CORIN can be shed into the circulation through autocleavage and metalloproteinase-mediated hydrolysis processes of the membrane form of the protein. Three different soluble fragments derived from corin molecules, with molecular masses of ~180, ~160 and ~100 kDa were described in literature (PMID: 21288900, 29387201).