COSMC Polyklonaler Antikörper

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COSMC Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19254-1-PBS

Synonyme

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  WB analysis of HeLa using 19254-1-AP (same clone as 19254-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 19254-1-AP (COSMC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 19254-1-AP (same clone as 19254-1-PBS)

  WB result of COSMC antibody (19254-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COSMC transfected HepG2 cells. This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 19254-1-AP (same clone as 19254-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19254-1-AP (COSMC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Caco-2 using 19254-1-AP (same clone as 19254-1-PBS)

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 19254-1-AP (COSMC antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 19254-1-AP (same clone as 19254-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 19254-1-AP (COSMC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 19254-1-AP (same clone as 19254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19254-1-AP (COSMC antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 19254-1-AP (same clone as 19254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19254-1-AP (COSMC antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19254-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19254-1-PBS bindet in WB, IHC, Indirect ELISA COSMC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COSMC fusion protein Ag6899
• Vollständiger Name: C1GALT1-specific chaperone 1
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36-37 kDa
• GenBank-Zugangsnummer: BC050441
• Gene symbol: COSMC
• Gene ID (NCBI): 29071
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COSMC (C1GALT1C1) is a molecular chaperone required for expression of active T-synthase (CIGALT1) which catalyzes the synthesis of Tn antigen. Tn antigens are cancer-associated glycans and play an important role in tumor progression. Dysfunctional COSMC is able to convert a wild type protein into a tumor-specific antigen affecting tumor cell biology. COSMC overexpression had been observed in various cancers. COSMC mutations have been observed in an autoimmune disease Tn syndrome.