COTL1 Monoklonaler Antikörper

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• KD/KO Validated

COTL1 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4A3D9

Kat-Nr. : 60237-1-PBS

Synonyme

CLP, Coactosin like protein, COTL1

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  WB analysis of A431 using 60237-1-Ig (same clone as 60237-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 60237-1-Ig (COTL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60237-1-Ig (same clone as 60237-1-PBS)

  WB result of COTL1 antibody (60237-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COTL1 transfected HeLa cells. This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 60237-1-Ig (same clone as 60237-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60237-1-Ig (COTL1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

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  WB analysis of human peripheral blood platelets using 60237-1-Ig (same clone as 60237-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 60237-1-Ig (COTL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60237-1-Ig (same clone as 60237-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60237-1-Ig (COTL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 60237-1-Ig (same clone as 60237-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60237-1-Ig (COTL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60237-1-Ig (same clone as 60237-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using COTL1 antibody (60237-1-Ig, Clone: 4A3D9 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60237-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60237-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA COTL1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: COTL1 fusion protein Ag1230
• Vollständiger Name: coactosin-like 1 (Dictyostelium)
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 14-16 kDa
• GenBank-Zugangsnummer: BC010884
• Gene symbol: COTL1
• Gene ID (NCBI): 23406
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COTL1, also named as CLP, binds to F-actin in a calcium-independent manner. And it has no direct effect on actin depolymerization.