SARS-CoV-2 S protein (RBD, 319-541 aa) Monoklonaler Antikörper

SARS-CoV-2 S protein (RBD, 319-541 aa) Monoklonal Antikörper für WB, Neutralization, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Virus und mehr (2)

Anwendung

WB, IF, Neutralization, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

CloneNo.

1H3E9

Kat-Nr. : 67758-1-Ig

Synonyme

COVID-19 RBD, RBD, Spike, spike protein, S protein



Geprüfte Anwendungen

Erfolgreiche Detektion in WBAg30688
Erfolgreiche NeutralizationRecombinant protein

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
NEUTRALIZATIONNEUTRALIZATION : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 9 publications below
IFSee 1 publications below

Produktinformation

67758-1-Ig bindet in WB, IF, Neutralization, ELISA SARS-CoV-2 S protein (RBD, 319-541 aa) und zeigt Reaktivität mit Virus

Getestete Reaktivität Virus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen SARS-CoV-2 S protein (RBD, 319-541 aa) fusion protein Ag30688
Vollständiger Name SARS-CoV-2 Spike Protein
Berechnetes Molekulargewicht 141 kDa
GenBank-ZugangsnummerNC_045512
Gene symbol SARS-COV-2
Gene ID (NCBI) 43740568
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616).

Lyophilized format of this product is available.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for SARS-CoV-2 S protein (RBD, 319-541 aa) antibody 67758-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Acta Pharm Sin B

A novel heterologous receptor-binding domain dodecamer universal mRNA vaccine against SARS-CoV-2 variants

Authors - Shugang Qin

J Med Virol

A novel three-plasmid packaging system for chimeric SFV/SIN VRPs derived from Semliki Forest virus and Sindbis virus as a candidate gene delivery vector

Authors - Yonghui Huang
humanWB

Biochem Pharmacol

Harringtonine: A more effective antagonist for Omicron variant

Authors - Shiling Hu

bioRxiv

Novel sensor-integrated proteome on chip (SPOC) platform with thousands of folded proteins on a 1.5 sq-cm biosensor chip to enable high-throughput real-time label-free screening for kinetic analysis

Authors - Chidozie Victor Agu
humanWB

Virol J

LINC01002 functions as a ceRNA to regulate FRMD8 by sponging miR-4324 for the development of COVID-19

Authors - Xinyi Kong
mouseWB

Int J Mol Sci

SARS-CoV-2 Rapidly Infects Peripheral Sensory and Autonomic Neurons, Contributing to Central Nervous System Neuroinvasion before Viremia

Authors - Jonathan D Joyce