COX17 Polyklonaler Antikörper

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• KD/KO Validated

COX17 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(20)

Kat-Nr. : 11464-1-AP

Synonyme

COX 17

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  WB analysis using 11464-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11464-1-AP (COX17 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 11464-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11464-1-AP (COX17 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 11464-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11464-1-AP (COX17 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11464-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11464-1-AP (COX17 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11464-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COX17 antibody (11464-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, Rattenherzgewebe, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Produktinformation

11464-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA COX17 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COX17 fusion protein Ag2032
• Vollständiger Name: COX17 cytochrome c oxidase assembly homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 63 aa, 7 kDa
• Beobachtetes Molekulargewicht: 7 kDa
• GenBank-Zugangsnummer: BC010933
• Gene symbol: COX17
• Gene ID (NCBI): 10063
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	Cell Stem Cell; Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.; Authors - Rashim Pal Singh ; KD Validated
human	IF	Am J Hum Genet; The mitochondrial disulfide relay system protein GFER is mutated in autosomal-recessive myopathy with cataract and combined respiratory-chain deficiency.; Authors - Di Fonzo Alessio A
human,rat	WB	Nutrients; Diosgenin Inhibits ROS Generation by Modulating NOX4 and Mitochondrial Respiratory Chain and Suppresses Apoptosis in Diabetic Nephropathy; Authors - Yujie Zhong
human,mouse	WB	FASEB J; Exercise improves angiogenic function of circulating exosomes in type 2 diabetes: Role of exosomal SOD3.; Authors - Kareem Abdelsaid
human	WB	J Biol Chem; An animal model for mitochondrial tyrosyl-tRNA synthetase deficiency reveals links between oxidative phosphorylation and retinal function.; Authors - Xiaofen Jin
mouse	WB	Toxicol Appl Pharmacol; Copper accumulation and compartmentalization in mouse fibroblast lacking metallothionein and copper chaperone, Atox1.; Authors - Miyayama Takamitsu T