COX2/ Cyclooxygenase 2/ PTGS2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

COX2/ Cyclooxygenase 2/ PTGS2 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

57

Rezensionen (1)

Kat-Nr. : 27308-1-AP

Synonyme

COX 2, COX2, COX2/ Cyclooxygenase 2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2

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Galerie der Validierungsdaten

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  WB analysis of A549 using 27308-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 27308-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 27308-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 27308-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 27308-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 27308-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse lung using 27308-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using COX2/ Cyclooxygenase 2/ PTGS2 antibody (27308-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse lung using 27308-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using COX2/ Cyclooxygenase 2/ PTGS2 antibody (27308-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 55 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

27308-1-AP bindet in WB, IP, IHC, IF, ELISA COX2/ Cyclooxygenase 2/ PTGS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag26174
• Vollständiger Name: prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
• Berechnetes Molekulargewicht: 604 aa, 68 kDa
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC013734
• Gene symbol: PTGS2
• Gene ID (NCBI): 5743
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa(PMID:19656660 ). It also expresses a band of 39 kDa after unspecific cleavage(PMID:17509125 ). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains(PMID:14724276 ).

Publikationen

Species	Application	Title
human,mouse	WB	Nucleic Acids Res; MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation; Authors - Bangming Jin
	WB	Small; M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation; Authors - Jie Sun
mouse	WB,IF	ACS Appl Mater Interfaces; Programmable Polymeric Microneedles for Combined Chemotherapy and Antioxidative Treatment of Rheumatoid Arthritis.; Authors - Chaoxiong Wu
mouse	IHC	Redox Biol; Topical hypochlorous acid (HOCl) blocks inflammatory gene expression and tumorigenic progression in UV-exposed SKH-1 high risk mouse skin.; Authors - Jana Jandova
mouse	WB,IHC	J Control Release; Combination of mitochondria impairment and inflammation blockade to combat metastasis; Authors - Xiaoli Yi
human	WB	Free Radic Biol Med; PET117 assembly factor stabilizes translation activator TACO1 thereby upregulates mitochondria-encoded cytochrome C oxidase 1 synthesis; Authors - Qiong Sun

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Ryan (Verified Customer) (02-27-2019)

Tissue was fixed in PFA with no additional antigen retrieval. Co-localisation with microglia based on known markers (not shown).

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish brain (Microglia) at 3dpf

COX2/ Cyclooxygenase 2/ PTGS2 Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (Microglia) at 3dpf (Cat no:27308-1-AP)