COX2/ Cyclooxygenase 2/ PTGS2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

COX2/ Cyclooxygenase 2/ PTGS2 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(114)

Rezensionen (2)

Kat-Nr. : 27308-1-AP

Synonyme

COX2, PTGS2, COX2/ Cyclooxygenase 2, Cyclooxygenase 2, Cyclooxygenase-2

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• 

  WB analysis using 27308-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27308-1-AP (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 27308-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 27308-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung using 27308-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 27308-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 27308-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 27308-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 27308-1-AP (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of mouse lung using 27308-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using COX2/ Cyclooxygenase 2/ PTGS2 antibody (27308-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse lung using 27308-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using COX2/ Cyclooxygenase 2/ PTGS2 antibody (27308-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Mauslungengewebe, THP-1-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungengewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:600-1:6000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 106 publications below
• IHC: See 12 publications below
• IF: See 14 publications below
• IP: See 1 publications below

Produktinformation

27308-1-AP bindet in WB, IHC, IF-P, IP, ELISA COX2/ Cyclooxygenase 2/ PTGS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag26174
• Vollständiger Name: prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
• Berechnetes Molekulargewicht: 604 aa, 68 kDa
• Beobachtetes Molekulargewicht: 69 kDa
• GenBank-Zugangsnummer: BC013734
• Gene symbol: COX2/PTGS2
• Gene ID (NCBI): 5743
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COX2 is an enzyme involved in the synthesis of prostaglandins from arachidonic acid.

1. What is the molecular weight of COX2?

The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also 

expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented 

PTGS2 has also previously been detected in AD brains (PMID:14724276).

2. Is COX2 post-translationally modified?

COX2 is a subject of post-translational modifications, including phosphorylation, glycosylation, and 

s-nitrosylation (PMID: 28939645).

3. What is the difference between COX1, COX2, and COX3?

There are three isoenzymes that have cyclooxygenase activity: COX1, COX2, and COX3. While COX1 

is a ubiquitously expressed constitutive enzyme, expression of COX2 is generally low but can be rapidly 

induced by various stimuli (as part of infection and inflammatory response) and is controlled by the 

transcription factor NFκB. COX3 is an alternative splice variant of COX1 enzyme and is considered 

non-functional in humans. 

4. I cannot detect COX2 in my sample during western blotting.

Unlike COX1, COX2 expression is inducible by a variety of stimuli including certain growth factors, cytokines, 

and proinflammatory stimuli. COX2 basal expression levels may be very low in unstimulated cells. Additionally, 

the COX2 half-life time is short and after stimulation, COX2 protein can be quickly degraded to basal levels 

(PMID: 10966456), which should be taken into account during experimental design.

5. What is the role of COX2 in cancer?

COX2 is often upregulated in various cancer types and increased expression of COX2 is associated with 

greater angiogenesis of solid tumors, increased invasion, and metastasis, as well as decreased host immunity.

6. What is subcellular localization of COX2?

Both COX1 and COX2 are present in the endoplasmic reticulum (ER) and nuclear envelope (PMID: 9545330), 

but COX2 has been reported to be more enriched in the nuclear envelope compared to COX1 (PMID: 7738031).

Publikationen

Species	Application	Title
mouse,human	WB	Nucleic Acids Res; MEN1 is a regulator of alternative splicing and prevents R-loop-induced genome instability through suppression of RNA polymerase II elongation; Authors - Bangming Jin
	WB	Small; M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation; Authors - Jie Sun
mouse	WB,IF	ACS Appl Mater Interfaces; Programmable Polymeric Microneedles for Combined Chemotherapy and Antioxidative Treatment of Rheumatoid Arthritis.; Authors - Chaoxiong Wu
mouse	IHC	Redox Biol; Topical hypochlorous acid (HOCl) blocks inflammatory gene expression and tumorigenic progression in UV-exposed SKH-1 high risk mouse skin.; Authors - Jana Jandova
mouse	WB	Int J Biol Macromol; Flammulina velutipes polysaccharide exerts immunomodulatory function involving RSAD2 to regulate the NF-κB/MAPK signaling pathway in RAW264.7 macrophage cells and in mouse spleen cells; Authors - Qiuyi Wang
mouse	WB,IHC	J Control Release; Combination of mitochondria impairment and inflammation blockade to combat metastasis; Authors - Xiaoli Yi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mei-xin (Verified Customer) (09-29-2025)

The antibody shows outstanding specificity, producing only one distinct band with no detectable background or additional bands.

• Applications: Western Blot

FH

Ryan (Verified Customer) (02-27-2019)

Tissue was fixed in PFA with no additional antigen retrieval. Co-localisation with microglia based on known markers (not shown).

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish brain (Microglia) at 3dpf

COX2/ Cyclooxygenase 2/ PTGS2 Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish brain (Microglia) at 3dpf (Cat no:27308-1-AP)