COX2/ Cyclooxygenase 2/ PTGS2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

COX2/ Cyclooxygenase 2/ PTGS2 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF-P, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(211)

CloneNo.

3G2B9

Kat-Nr. : 66351-1-Ig

Synonyme

COX2, PTGS2, 3G2B9, EC:1.14.99.1, Cyclooxygenase-2

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  WB analysis using 66351-1-Ig

  Non-treated and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66351-1-Ig

  Untreated and LPS-treated RAW264.7 cells were subjected to SDS PAGE followed by western blot with 66351-1-Ig (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66351-1-Ig

  Untreated and LPS-treated Hela cells were subjected to SDS PAGE followed by western blot with 66351-1-Ig (COX2/ Cyclooxygenase 2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 66351-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66351-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66351-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66351-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66351-1-Ig (COX2/ Cyclooxygenase 2/ PTGS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse lung using 66351-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using COX2/ Cyclooxygenase 2/ PTGS2 antibody (66351-1-Ig, Clone: 3G2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse lung using 66351-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using COX2/ Cyclooxygenase 2/ PTGS2 antibody (66351-1-Ig, Clone: 3G2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, mit LPS behandelte HeLa-Zellen, mit LPS behandelte RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslungengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 179 publications below
• IHC: See 28 publications below
• IF: See 23 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

66351-1-Ig bindet in WB, IHC, IF-P, IP, CoIP, ELISA COX2/ Cyclooxygenase 2/ PTGS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: COX2/ Cyclooxygenase 2/ PTGS2 fusion protein Ag24721
• Vollständiger Name: prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
• Berechnetes Molekulargewicht: 604 aa, 68 kDa
• Beobachtetes Molekulargewicht: 66-70 kDa
• GenBank-Zugangsnummer: BC013734
• Gene symbol: COX2/PTGS2
• Gene ID (NCBI): 5743
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COX2 is an enzyme involved in the synthesis of prostaglandins from arachidonic acid.

1. What is the molecular weight of COX2?

The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also 

expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented 

PTGS2 has also previously been detected in AD brains (PMID:14724276).

2. Is COX2 post-translationally modified?

COX2 is a subject of post-translational modifications, including phosphorylation, glycosylation, and 

s-nitrosylation (PMID: 28939645).

3. What is the difference between COX1, COX2, and COX3?

There are three isoenzymes that have cyclooxygenase activity: COX1, COX2, and COX3. While COX1 

is a ubiquitously expressed constitutive enzyme, expression of COX2 is generally low but can be rapidly 

induced by various stimuli (as part of infection and inflammatory response) and is controlled by the 

transcription factor NFκB. COX3 is an alternative splice variant of COX1 enzyme and is considered 

non-functional in humans. 

4. I cannot detect COX2 in my sample during western blotting.

Unlike COX1, COX2 expression is inducible by a variety of stimuli including certain growth factors, cytokines, 

and proinflammatory stimuli. COX2 basal expression levels may be very low in unstimulated cells. Additionally, 

the COX2 half-life time is short and after stimulation, COX2 protein can be quickly degraded to basal levels 

(PMID: 10966456), which should be taken into account during experimental design.

5. What is the role of COX2 in cancer?

COX2 is often upregulated in various cancer types and increased expression of COX2 is associated with 

greater angiogenesis of solid tumors, increased invasion, and metastasis, as well as decreased host immunity.

6. What is subcellular localization of COX2?

Both COX1 and COX2 are present in the endoplasmic reticulum (ER) and nuclear envelope (PMID: 9545330), 

but COX2 has been reported to be more enriched in the nuclear envelope compared to COX1 (PMID: 7738031).

Publikationen

Species	Application	Title
human	WB	Cell Metab; Cooperation between COA6 and SCO2 in COX2 maturation during cytochrome c oxidase assembly links two mitochondrial cardiomyopathies.; Authors - David Pacheu-Grau
	IHC	ACS Nano; Engineered Extracellular Vesicle-Delivered CRISPR/Cas9 for Radiotherapy Sensitization of Glioblastoma; Authors - Xiao Liu
mouse	WB	Adv Sci (Weinh); Tryptophan As a New Member of RNA-Induced Silencing Complexes Prevents Colon Cancer Liver Metastasis; Authors - Fangyi Xu
human	WB	Redox Biol; The deubiquitinase USP11 ameliorates intervertebral disc degeneration by regulating oxidative stress-induced ferroptosis via deubiquitinating and stabilizing Sirt3; Authors - Jian Zhu
mouse	WB	J Nanobiotechnology; Injectable photocrosslinking spherical hydrogel-encapsulated targeting peptide-modified engineered exosomes for osteoarthritis therapy; Authors - Junlai Wan
mouse	IHC	J Nanobiotechnology; Low-dose celecoxib-loaded PCL fibers reverse intervertebral disc degeneration by up-regulating CHSY3 expression; Authors - Yunhao Wang