COX4I2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

COX4I2 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

16

Kat-Nr. : 11463-1-AP

Synonyme

COX IV 2, COX4, COX4 2, COX4B, COX4I2, COX4L2, COXIV 2

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• Galerie der Validierungsdaten
• Proteintech vs. Other Vendors

• 

  WB analysis using 11463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

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  WB analysis of human brain using 11463-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11463-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 11463-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11463-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11463-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11463-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11463-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 11463-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment using 11463-1-AP

  IP result of anti-COX4I2 (11463-1-AP for IP and Detection) with HepG2 cell lysate.

• 

  IP experiment of HepG2 using 11463-1-AP

  IP Result of anti-COX4I2 (IP:11463-1-AP, 3ug; Detection:11463-1-AP 1:1000) with HepG2 cells lysate 8000ug.

• 

  IHC staining of human ovary tumor using 11463-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11463-1-AP (COX4I2 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 11463-1-AP

  Immunofluorescent analysis of Hela cells, using COX4I2 antibody 11463-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A375-Zellen, humanes Herzgewebe, humanes Hirngewebe, humanes Lungengewebe, MCF-7-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Produktinformation

11463-1-AP bindet in WB, IP, IHC, IF, ELISA COX4I2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COX4I2 fusion protein Ag1987
• Vollständiger Name: cytochrome c oxidase subunit IV isoform 2 (lung)
• Berechnetes Molekulargewicht: 171 aa, 20 kDa
• Beobachtetes Molekulargewicht: 17-18 kDa
• GenBank-Zugangsnummer: BC057779
• Gene symbol: COX4I2
• Gene ID (NCBI): 84701
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COX4I2, also named as COX4L2 and COXIV-2, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COXIV(cytochrome c oxidase IV) has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. This antibody was generated against full length COX4I2 protein.

Publikationen

Species	Application	Title
mouse	WB	Cell Rep; Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1α Expression in Hypoxia.; Authors - Arpita Chowdhury
human	WB	Oncotarget; Nuclear-encoded cytochrome c oxidase subunit 4 regulates BMI1 expression and determines proliferative capacity of high-grade gliomas.; Authors - Claudia R Oliva ; KD Validated
mouse	IF	Sci Signal; Acute O2 sensing through HIF2α-dependent expression of atypical cytochrome oxidase subunits in arterial chemoreceptors.; Authors - Alejandro Moreno-Domínguez ; KO Validated
human	WB	Free Radic Biol Med; Cytochrome c oxidase mediates labile iron level and radioresistance in glioblastoma.; Authors - Md Yousuf Ali
human	WB	Bioconjug Chem; GnRH-Gemcitabine Conjugates for the Treatment of Androgen-Independent Prostate Cancer: Pharmacokinetic Enhancements Combined with Targeted Drug Delivery.; Authors - Theodoros Karampelas
human	WB	Free Radic Biol Med; Differentiation of SH-SY5Y cells to a neuronal phenotype changes cellular bioenergetics and the response to oxidative stress.; Authors - Schneider Lonnie L