COX4I2 Polyklonaler Antikörper

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• KD/KO Validated

COX4I2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11463-1-PBS

Synonyme

COX4L2, COX4B, COX4 2, COX4, COX IV-2

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  WB analysis using 11463-1-AP (same clone as 11463-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1500 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 11463-1-AP (same clone as 11463-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 11463-1-AP (same clone as 11463-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 11463-1-AP (same clone as 11463-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 11463-1-AP (same clone as 11463-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 11463-1-AP (same clone as 11463-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 11463-1-AP (same clone as 11463-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 11463-1-AP (same clone as 11463-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

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  WB analysis of human lung using 11463-1-AP (same clone as 11463-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 11463-1-AP (COX4I2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

• 

  IP experiment using 11463-1-AP (same clone as 11463-1-PBS)

  IP result of anti-COX4I2 (11463-1-AP for IP and Detection) with HepG2 cell lysate. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 11463-1-AP (same clone as 11463-1-PBS)

  IP result of anti-COX4I2 (IP:11463-1-AP, 3ug; Detection:11463-1-AP 1:1000) with HepG2 cells lysate 8000ug. This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 11463-1-AP (same clone as 11463-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11463-1-AP (COX4I2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11463-1-AP (same clone as 11463-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COX4I2 antibody (11463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 11463-1-AP (same clone as 11463-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COX4I2 antibody (11463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 11463-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11463-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA COX4I2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COX4I2 fusion protein Ag1987
• Vollständiger Name: cytochrome c oxidase subunit IV isoform 2 (lung)
• Berechnetes Molekulargewicht: 171 aa, 20 kDa
• Beobachtetes Molekulargewicht: 17-18 kDa
• GenBank-Zugangsnummer: BC057779
• Gene symbol: COX4I2
• Gene ID (NCBI): 84701
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COX4I2, also named as COX4L2 and COXIV-2, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COXIV(cytochrome c oxidase IV) has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. This antibody was generated against full length COX4I2 protein.