COX6A1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

COX6A1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Kat-Nr. : 11460-1-AP

Synonyme

COX VIa L, COX VIa-L, COX6AL, Cytochrome c oxidase polypeptide VIa-liver, Cytochrome c oxidase subunit VIA-liver

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  WB analysis using 11460-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11460-1-AP (COX6A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 11460-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11460-1-AP (COX6A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11460-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11460-1-AP (COX6A1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas cancer using 11460-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11460-1-AP (COX6A1 Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of MCF-7 using 11460-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using COX6A1 antibody (11460-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of SH-SY5Y using 11460-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 11460-1-AP (COX6A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, NIH/3T3-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IF: See 1 publications below

Produktinformation

11460-1-AP bindet in WB, IHC, IF/ICC, ELISA COX6A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COX6A1 fusion protein Ag2006
• Vollständiger Name: cytochrome c oxidase subunit VIa polypeptide 1
• Berechnetes Molekulargewicht: 85 aa, 9 kDa
• Beobachtetes Molekulargewicht: 9.5 kDa
• GenBank-Zugangsnummer: BC007723
• Gene symbol: COX6A1
• Gene ID (NCBI): 1337
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COX6A1(Cytochrome c oxidase subunit 6A1, mitochondrial) is also named as COX6AL and belongs to the cytochrome c oxidase subunit 6A family.This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

Publikationen

Species	Application	Title
mouse,human	WB,IF	Autophagy; PLK1 (polo like kinase 1)-dependent autophagy facilitates gefitinib-induced hepatotoxicity by degrading COX6A1 (cytochrome c oxidase subunit 6A1).; Authors - Peihua Luo ; KD Validated
human	WB	Elife; Function of hTim8a in complex IV assembly in neuronal cells provides insight into pathomechanism underlying Mohr-Tranebjærg syndrome.; Authors - Yilin Kang
human	WB	Cell Death Dis; O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.; Authors - Weicheng Wu
human	WB	Mol Cell Proteomics; HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV; Authors - Daniella H Hock
mouse	WB	Hum Mol Genet; Pathological characterization of a novel mouse model expressing the PD-linked CHCHD2-T61I mutation.; Authors - Teresa R Kee
human	WB	Mol Cell Proteomics; HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV.; Authors - Daniella H Hock