COX7C Polyklonaler Antikörper

COX7C Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 11411-2-AP

Synonyme

COX7C

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Galerie der Validierungsdaten

• 

  WB analysis of mouse ovary using 11411-2-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11411-2-AP (COX7C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 11411-2-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11411-2-AP (COX7C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 11411-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11411-2-AP (COX7C antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of MCF-7 using 11411-2-AP

  Immunofluorescent analysis of MCF-7 cells, using COX7C antibody 11411-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Eierstockgewebe, humanes Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 2 publications below

Produktinformation

11411-2-AP bindet in WB, IHC, IF, ELISA COX7C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COX7C fusion protein Ag1989
• Vollständiger Name: cytochrome c oxidase subunit VIIc
• Berechnetes Molekulargewicht: 63 aa, 7 kDa
• Beobachtetes Molekulargewicht: 15~28 kDa
• GenBank-Zugangsnummer: BC007498
• Gene symbol: COX7C
• Gene ID (NCBI): 1350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COX7C(Cytochrome c oxidase subunit 7C, mitochondrial) is the terminal enzyme in the mitochondrial respiratory chain (oxidative phosphorylation OXPHOS), catalyzing the elctron transfer from reduced cytochrome c to molecule oxygen. This protein belongs to the cytochrome c oxidase VIIc family. The full length protein has a transit peptide with 16 amino acids. This antibody is specific to COX7C.

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.; Authors - Weicheng Wu
human	WB	J Biol Chem; PMID: 20870728; Authors - Oliva Claudia R CR
human	WB	Cancer Sci; Urea transport B gene induces melanoma B16 cell death via activation of p53 and mitochondrial apoptosis.; Authors - Lianqin Liu
human	IF	J Neuropathol Exp Neurol; Altered Mitochondria, Protein Synthesis Machinery, and Purine Metabolism Are Molecular Contributors to the Pathogenesis of Creutzfeldt-Jakob Disease.; Authors - Belén Ansoleaga
mouse	WB,IF	Front Pharmacol; Effect of Dl-3-n-butylphthalide on mitochondrial Cox7c in models of cerebral ischemia/reperfusion injury; Authors - Jingjing Jia ; KD Validated
mouse	WB	Nat Commun; ZHX2 emerges as a negative regulator of mitochondrial oxidative phosphorylation during acute liver injury; Authors - Yankun Zhang