COX8A Polyklonaler Antikörper

COX8A Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Rezensionen (1)

Kat-Nr. : 15368-1-AP

Synonyme

COX8, COX8 2, COX8L, Cytochrome c oxidase polypeptide VIII-liver/heart, Cytochrome c oxidase subunit 8-2

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  WB analysis of mouse skeletal muscle using 15368-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15368-1-AP (COX8A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 15368-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15368-1-AP (COX8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat heart using 15368-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15368-1-AP (COX8A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 15368-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15368-1-AP (COX8A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15368-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15368-1-AP (COX8A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 15368-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15368-1-AP (COX8A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 15368-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15368-1-AP (COX8A antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, humanes Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe, humanes Leberzirrhosegewebe, Rattenherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15368-1-AP bindet in WB, IHC, IF, ELISA COX8A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: COX8A fusion protein Ag2885
• Vollständiger Name: cytochrome c oxidase subunit 8A (ubiquitous)
• Berechnetes Molekulargewicht: 8 kDa
• Beobachtetes Molekulargewicht: 8 kDa
• GenBank-Zugangsnummer: BC063025
• Gene symbol: COX8A
• Gene ID (NCBI): 1351
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COX8A(Cytochrome c oxidase subunit 8A) is also named as COX8, COX8L and belongs to the cytochrome c oxidase VIII family. This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. The gene encodes a full length protein of 8 kDa with a transit peptide of 25 amino acid.

Publikationen

Species	Application	Title
human	WB	Cell Death Dis; O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.; Authors - Weicheng Wu
human	IHC	J Proteomics; MALDI-MS tissue imaging identification of biliverdin reductase B overexpression in prostate cancer.; Authors - Pallua Johannes Dominikus JD
mouse	WB	Nat Commun; A FRET-based respirasome assembly screen identifies spleen tyrosine kinase as a target to improve muscle mitochondrial respiration and exercise performance in mice; Authors - Ami Kobayashi
rat	WB	Basic Res Cardiol; Comparison of the stage-dependent mitochondrial changes in response to pressure overload between the diseased right and left ventricle in the rat; Authors - Ling Li
mouse	WB,IF	J Adv Res; Lactational high weight loss impairs follicular development by causing mitochondrial dysfunction of ovarian cells in sows and mitigated by butyrate supplement; Authors - Kexiong Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Svitlana (Verified Customer) (03-01-2019)

HEK293t transfected with Cox8-eGFP, 48 hours post-transfection cells collected for mitochondria isolation. 10 ug mitochondrial lysate (in RIPA buffer) was loaded per line.Incubation with primary anti-Cox8 antibody overnight at 4C.Image: 1st line protein ladder, 2nd line no-transfection sample, 3rd line Cox8-eGFP transfected sample.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293t

COX8A Antibody Western Blot, validation (1:1000 dilution) in HEK293t (Cat no:15368-1-AP)