Carboxypeptidase A1 Polyklonaler Antikörper

Carboxypeptidase A1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Rezensionen (1)

Kat-Nr. : 15836-1-AP

Synonyme

CPA1, EC:3.4.17.1, CPA

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  WB analysis of mouse pancreas using 15836-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 15836-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15836-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15836-1-AP (Carboxypeptidase A1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of U2OS using 15836-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using Carboxypeptidase A1 antibody (15836-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Nierengewebe, humanes Lebergewebe, humanes Lungengewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 5 publications below

Produktinformation

15836-1-AP bindet in WB, IHC, IF/ICC, ELISA Carboxypeptidase A1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Carboxypeptidase A1 fusion protein Ag8586
• Vollständiger Name: carboxypeptidase A1 (pancreatic)
• Berechnetes Molekulargewicht: 419 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC005279
• Gene symbol: Carboxypeptidase A1
• Gene ID (NCBI): 1357
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPA1(Carboxypeptidase A1) is also named as CPA and belongs to the the peptidase M14 family. CPA1 is one of the genes whose expression in the pancreas and the protein which it encodes is synthesized as an inactive precursor, proCPA1, which is processed to the active enzyme by the proteolytic removal of the 95-amino acid N-terminal prodomain. The mature CPA1 is not secreted and is trapped and degraded intracellularly(PMID:8639628). This antibody is speicific to CPA1.

Publikationen

Species	Application	Title
mouse	IF	Cell Rep; Spatiotemporal role of SETD2-H3K36me3 in murine pancreatic organogenesis; Authors - Ping Lu
mouse	IHC	Cell Rep; αE-Catenin Is a Positive Regulator of Pancreatic Islet Cell Lineage Differentiation.; Authors - Antonio J Jimenez-Caliani
mouse	IF	Development; Focal adhesion kinase-mediated signaling controls the onset of pancreatic cell differentiation; Authors - Uylissa A Rodriguez
rat	IF	J Nutr Biochem; Intermittent protein restriction improves glucose homeostasis in Zucker diabetic fatty rats and single-cell sequencing reveals distinct changes in β cells; Authors - Chenchen Li
mouse	IF	Pancreatology; Smad4 deficiency substitutes Cdkn2b but not Cdkn2a downregulation in pancreatic cancer following induction of genetic events in adult mice.; Authors - Xintong Jia
	IF	Dis Model Mech; A novel pancreatic cancer model originated from transformation of acinar cells in adult tree shrew, a primate-like animal.; Authors - Qiu Tu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (02-14-2025)

Carboxypeptidase A1 (in green) seemed to work well on my human brain FFPE tissue. It does not mark my neurons (in red), but the possible mast cells in the epileptic cortex.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

Carboxypeptidase A1 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:15836-1-AP)