CPA1 Monoklonaler Antikörper

CPA1 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

2H2E8

Kat-Nr. : 68826-1-PBS

Synonyme

Carboxypeptidase A1, 2H2E8, CPA, EC:3.4.17.1

---

• 

  WB analysis using 68826-1-Ig (same clone as 68826-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68826-1-Ig (CPA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68826-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 68826-1-Ig (same clone as 68826-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68826-1-Ig (CPA1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68826-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 68826-1-Ig (same clone as 68826-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68826-1-Ig (CPA1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68826-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 68826-1-Ig (same clone as 68826-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 68826-1-Ig (CPA1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68826-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 68826-1-Ig (same clone as 68826-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 68826-1-Ig (CPA1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68826-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pancreas using 68826-1-Ig (same clone as 68826-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CPA1 antibody (68826-1-Ig, Clone: 2H2E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68826-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

68826-1-PBS bindet in WB, IHC, IF-P, ELISA CPA1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CPA1 fusion protein Ag8579
• Vollständiger Name: carboxypeptidase A1 (pancreatic)
• Berechnetes Molekulargewicht: 419 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC005279
• Gene symbol: Carboxypeptidase A1
• Gene ID (NCBI): 1357
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPA1(Carboxypeptidase A1) is also named as CPA and belongs to the the peptidase M14 family. CPA1 is one of the genes whose expression in the pancreas and the protein which it encodes is synthesized as an inactive precursor, proCPA1, which is processed to the active enzyme by the proteolytic removal of the 95-amino acid N-terminal prodomain. The mature CPA1 is not secreted and is trapped and degraded intracellularly(PMID:8639628). This antibody is speicific to CPA1.