Carboxypeptidase A6 Polyklonaler Antikörper

Carboxypeptidase A6 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 13604-1-AP

Synonyme

Carboxypeptidase A6, Carboxypeptidase B, CPA6, CPAH

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 13604-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13604-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13604-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13604-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 13604-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13604-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13604-1-AP

  Immunofluorescent analysis of HepG2 cells using 13604-1-AP (Carboxypeptidase A6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

13604-1-AP bindet in WB, IHC, IF, ELISA Carboxypeptidase A6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Carboxypeptidase A6 fusion protein Ag4466
• Vollständiger Name: carboxypeptidase A6
• Berechnetes Molekulargewicht: 437 aa, 51 kDa
• Beobachtetes Molekulargewicht: 65 kDa, 51 kDa
• GenBank-Zugangsnummer: BC033684
• Gene symbol: CPA6
• Gene ID (NCBI): 57094
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Carboxypeptidase A6 (CPA6) is an extracellular matrixbound metallocarboxypeptidase (CP) that has been implicated in Duane syndrome, a neurodevelopmental disorder in which the lateral rectus extraocularmuscle is not properly innervated.Consistent with a role inDuane syndrome, CPA6 is expressed in a number of chondrocytic and nervous tissues during embryogenesis.It has three isoforms.The product recognizes the C-terminal of this protein.

Publikationen

Species	Application	Title
human	WB	Arch Biochem Biophys; Carboxypeptidase A6 suppresses the proliferation and invasion of colorectal cancer cells and is negatively regulated by miR-96-3p; Authors - Xianren Wang