Carboxypeptidase B2 Polyklonaler Antikörper

Carboxypeptidase B2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 10672-1-AP

Synonyme

Carboxypeptidase B2, carboxypeptidase B2 (plasma), Carboxypeptidase U, CPB2, CPU, PCPB, Plasma carboxypeptidase B, TAFI

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  WB analysis of mouse liver using 10672-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10672-1-AP (Carboxypeptidase B2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 10672-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10672-1-AP (Carboxypeptidase B2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 10672-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10672-1-AP (Carboxypeptidase B2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10672-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10672-1-AP (Carboxypeptidase B2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

10672-1-AP bindet in WB, IHC, ELISA Carboxypeptidase B2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Carboxypeptidase B2 fusion protein Ag1110
• Vollständiger Name: carboxypeptidase B2 (plasma)
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC007057
• Gene symbol: Carboxypeptidase B2
• Gene ID (NCBI): 1361
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPB2(Carboxypeptidase B2) belongs to the peptidase M14 family. The CPB2 gene encoding TAFI is a candidate gene for blood pressure. Circulating CPB2 and platelets may potentially contribute to regulating the bioactivity of leukocyte chemoattractant chemerin (RARRES2), and further extend the molecular link between blood coagulation/fibrinolysis and CMKLR1-mediated immune responses. This protein has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse	WB	FEMS Immunol Med Microbiol; Secreted Bacillus anthracis proteases target the host fibrinolytic system.; Authors - Chung Myung-Chul MC
mouse	WB	Neurochem Res; Serum Proteomics Identified TAFI as a Potential Molecule Facilitating the Migration of Peripheral Monocytes to Damaged White Matter During Chronic Cerebral Hypoperfusion; Authors - Yuhan Wang
rat	IHC	Int J Biol Macromol; Multi-omics analysis reveals the pre-protective mechanism of Dendrobium flexicaule polysaccharide against alcohol-induced gastric mucosal injury; Authors - Fei Wang