CPE Rekombinanter Antikörper

CPE Rekombinant Antikörper für WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250680F12

Kat-Nr. : 86111-1-PBS

Synonyme

Carboxypeptidase E, Carboxypeptidase H, CPH, EC:3.4.17.10, Prohormone-processing carboxypeptidase

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  WB analysis using 86111-1-RR (same clone as 86111-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86111-1-RR (CPE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86111-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02472-1

  Sandwich ELISA standard curve of MP02472-1, Human CPE Recombinant Matched Antibody Pair - PBS only. 86111-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2989. 86111-1-PBS was HRP conjugated as the detection antibody. Range: 1.56-50 ng/mL

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  Sample test of MP02472-1

  Serum of fifteen individual healthy human donors was measured. The human CPE concentration of detected samples was determined to be 5.22 ng/mL with a range of 0.76 - 15.81 ng/mL.

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  IHC staining of mouse brain using 86111-1-RR (same clone as 86111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86111-1-RR (CPE antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86111-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 86111-1-RR (same clone as 86111-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86111-1-RR (CPE antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86111-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-12 using 86111-1-RR (same clone as 86111-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using CPE antibody (86111-1-RR, Clone: 250680F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86111-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

86111-1-PBS bindet in WB, IHC, IF/ICC, Sandwich ELISA, Indirect ELISA, Sample test CPE und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CPE fusion protein Eg2989
• Vollständiger Name: carboxypeptidase E
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: NM_001873.4
• Gene symbol: Carboxypeptidase E
• Gene ID (NCBI): 1363
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.