CPEB4 Polyklonaler Antikörper

CPEB4 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 25342-1-AP

Synonyme

CPE binding protein 4, CPE BP4, CPEB4, hCPEB 4, KIAA1673

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Galerie der Validierungsdaten

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  WB analysis of SH-SY5Y using 25342-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25342-1-AP (CPEB4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25342-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25342-1-AP (CPEB4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 25342-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 25342-1-AP (CPEB4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 25342-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25342-1-AP (CPEB4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 25342-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25342-1-AP (CPEB4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 25342-1-AP

  Immunofluorescent analysis of (-20℃ Acetone) fixed SH-SY5Y cells using 25342-1-AP (CPEB4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, A375-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below
• RIP: See 1 publications below

Produktinformation

25342-1-AP bindet in WB, RIP, IHC, IF, ELISA CPEB4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: cytoplasmic polyadenylation element binding protein 4
• Berechnetes Molekulargewicht: 80 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: NM_030627
• Gene symbol: CPEB4
• Gene ID (NCBI): 80315
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPEB4 also named as CPE binding protein 4 or hCPEB 4 is a 729 amino acid protein, which contains two RRM domains and belongs to the RRM CPEB family. CPEB4 involves in some forms of synaptic plasticity and regulate translation by binding to regulatory motifs of defined mRNA targets. CPEB1 and CPEB4 are differentially regulated by phase-specific kinases, generating the need of two sequential CPEB activities to sustain cytoplasmic polyadenylation during all the meiotic phases.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation.; Authors - Kai P Hoefig
human	WB	Front Cell Dev Biol; CPEB4 Inhibit Cell Proliferation via Upregulating p21 mRNA Stability in Renal Cell Carcinoma.; Authors - Jiehui Di
human	WB,IHC	J Transl Med; CircESRP1 enhances metastasis and epithelial-mesenchymal transition in endometrial cancer via the miR-874-3p/CPEB4 axis.; Authors - Rui Shi
human	WB	Technol Cancer Res Treat; miRNA-130a-3p/CPEB4 Axis Modulates Glioblastoma Growth and Progression; Authors - Hongchao Liu
mouse	WB	Acta Cir Bras; Gynura segetum induces hepatic sinusoidal obstruction syndrome in mice by impairing autophagy.; Authors - Hui Zhang
mouse	WB	bioRxiv; Inhibition of CPEB3 ribozyme elevates CPEB3 protein expression and polyadenylation of its target mRNAs, and enhances object location memory; Authors - Claire C Chen