CPLX1 Rekombinanter Antikörper

CPLX1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250131A9

Kat-Nr. : 85874-1-RR

Synonyme

Complexin-1, CPLX 1, CPX I, CPX1, CPXI

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  WB analysis of SH-SY5Y using 85874-1-RR

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 85874-1-RR (CPLX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85874-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85874-1-RR (CPLX1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85874-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85874-1-RR against Human CPLX1 were performed. The affinity constant is 0.717 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe, Ratten-Cerebellum-Gewebe, SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85874-1-RR bindet in WB, ELISA CPLX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CPLX1 fusion protein Ag0387
• Vollständiger Name: complexin 1
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 15-20 kDa
• GenBank-Zugangsnummer: BC002471
• Gene symbol: CPLX1
• Gene ID (NCBI): 10815
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Complexin 1 (CPLX1) is a member of the complexin/synaphin gene family, which are cytosolic proteins that function in synaptic vesicle exocytosis. Complexins are enriched in neurons where they colocalize with syntaxin and SNAP-25. Complexins bind weakly to syntaxin alone and not at all to synaptobrevin and SNAP-25, but strongly to the SNAP receptor-core complex composed of these three molecules. Compexins also compete with alpha-SNAP for binding to the core complex but not with other interacting molecules, including synaptotagmin I, and regulate the sequential interactions of alpha-SNAP and synaptotagmins with the SNAP receptor during exocytosis. CLPX1 binds to the SNAP receptor complex and disrupts it, leading to the release of transmitters. Alterations of complexins may contribute to the molecular substrate for abnormalities of neural connectivity in severe mental disorders.