CPLX2 Polyklonaler Antikörper

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• KD/KO Validated

CPLX2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18149-1-PBS

Synonyme

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  WB analysis of mouse brain using 18149-1-AP (same clone as 18149-1-PBS)

  mouse and rat brain tissue were subjected to SDS PAGE followed by western blot with 18149-1-AP (CPLX2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18149-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 18149-1-AP (same clone as 18149-1-PBS)

  WB result of CPLX2 antibody (18149-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPLX2 transfected A549 cells. This data was developed using the same antibody clone with 18149-1-PBS in a different storage buffer formulation.

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  IP experiment of A549 using 18149-1-AP (same clone as 18149-1-PBS)

  IP result of anti-CPLX2 (IP:18149-1-AP, 4ug; Detection:18149-1-AP 1:1000) with A549 cells lysate 600 ug. This data was developed using the same antibody clone with 18149-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 18149-1-AP (same clone as 18149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18149-1-AP (CPLX2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18149-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 18149-1-AP (same clone as 18149-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 18149-1-AP (CPLX2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18149-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 18149-1-AP (same clone as 18149-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CPLX2 antibody (18149-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 18149-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18149-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CPLX2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPLX2 fusion protein Ag12838
• Vollständiger Name: complexin 2
• Berechnetes Molekulargewicht: 134 aa, 15 kDa
• Beobachtetes Molekulargewicht: 18-20 kDa
• GenBank-Zugangsnummer: BC093706
• Gene symbol: CPLX2
• Gene ID (NCBI): 10814
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Complexins are soluble proteins that regulate the activity of soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complexes necessary for vesicle fusion. Neuronal specific complexin 1 (CPLX1) has inhibitory and stimulatory effects on exocytosis by clamping trans-SNARE complexes in a prefusion state and promoting conformational changes to facilitate membrane fusion following cell stimulation. Complexin2 (CPLX2) is a pre-synaptic protein believed to regulate neurotransmitter release from pre-synaptic terminals, it is downregulated in schizophrenic patients suffering from depression, animal models of depression and neurological disorders such as Huntington's disease in which depression is a major symptom.