Carboxypeptidase M Polyklonaler Antikörper

Carboxypeptidase M Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 16440-1-AP

Synonyme

Carboxypeptidase M, CPM

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  WB analysis of HEK-293 using 16440-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16440-1-AP (Carboxypeptidase M antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16440-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16440-1-AP (Carboxypeptidase M antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16440-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16440-1-AP (Carboxypeptidase M antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

16440-1-AP bindet in WB, ELISA Carboxypeptidase M und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Carboxypeptidase M fusion protein Ag9761
• Vollständiger Name: carboxypeptidase M
• Berechnetes Molekulargewicht: 443 aa, 51 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC022276
• Gene symbol: Carboxypeptidase M
• Gene ID (NCBI): 1368
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPM(Carboxypeptidase M), a plasma membrane-bound enzyme, is present in many human organs and differs from other carboxypeptidases that cleave basic C-terminal amino acids(PMID:2394713). CPM is structurally, catalytically and immunologically distinct from other carboxypeptidases, and expressed on cell membranes in a glycosyl-phosphatidylinositol-anchored form and it can be a novel marker and cellular player in lipid uptake and/or metabolism of MAs by promoting foam cell formation (PMID:22157720). This full length protein has a signal peptide with 17 amino acid, a propeptide with 20 amino acid and five glycosylation sites.

Publikationen

Species	Application	Title
human	WB	Sci Adv; FAM50A drives breast cancer brain metastasis through interaction with C9ORF78 to enhance ʟ-asparagine production; Authors - Weilong Chen