CPN1 Polyklonaler Antikörper

CPN1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13385-1-PBS

Synonyme

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  WB analysis of Raji using 13385-1-AP (same clone as 13385-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 13385-1-AP (CPN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

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  WB analysis using 13385-1-AP (same clone as 13385-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13385-1-AP (CPN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 13385-1-AP (same clone as 13385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13385-1-AP (CPN1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 13385-1-AP (same clone as 13385-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 13385-1-AP (CPN1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

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  IF Staining of COLO 320 using 13385-1-AP (same clone as 13385-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using CPN1 antibody (13385-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 13385-1-AP (same clone as 13385-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CPN1 antibody (13385-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 13385-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13385-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CPN1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPN1 fusion protein Ag4035
• Vollständiger Name: carboxypeptidase N, polypeptide 1
• Berechnetes Molekulargewicht: 458 aa, 52 kDa
• Beobachtetes Molekulargewicht: 40~50 kDa
• GenBank-Zugangsnummer: BC027897
• Gene symbol: CPN1
• Gene ID (NCBI): 1369
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. Normally, CPN circulates in blood plasma as a hetero-tetramer consisting of two 83 kDa (CPN2) domains each flanked by a 48 to 55 kDa catalytic (CPN1) domain. The C terminal region of the CPN1 subunit differs from all other family members in that it contains numerous basic residues. Proteolysis at these sites apparently happens either constitutively in the blood or during processing and secretion from the liver.