CPN1 Rekombinanter Antikörper

CPN1 Rekombinant Antikörper für WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241481A5

Kat-Nr. : 84218-1-PBS

Synonyme

241481A5, ACBP, Arginine carboxypeptidase, CPN, EC:3.4.17.3

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  WB analysis of human plasma using 84218-1-RR (same clone as 84218-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 84218-1-RR (CPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84218-1-RR (same clone as 84218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 84218-1-RR (same clone as 84218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 84218-1-RR (same clone as 84218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 84218-1-RR (same clone as 84218-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 84218-1-RR (same clone as 84218-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CPN1 antibody (84218-1-RR, Clone: 241481A5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84218-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01152-1

  Cytometric bead array standard curve of MP01152-1, CPN1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84218-2-PBS. Detection antibody: 84218-1-PBS. Standard: Ag4035. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84218-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 84218-1-PBS against Human CPN1 was performed. The affinity constant is 12.4 nM.

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Geprüfte Anwendungen

Produktinformation

84218-1-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA CPN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CPN1 fusion protein Ag4035
• Vollständiger Name: carboxypeptidase N, polypeptide 1
• Berechnetes Molekulargewicht: 458 aa, 52 kDa
• Beobachtetes Molekulargewicht: 40~50 kDa
• GenBank-Zugangsnummer: BC027897
• Gene symbol: CPN1
• Gene ID (NCBI): 1369
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. Normally, CPN circulates in blood plasma as a hetero-tetramer consisting of two 83 kDa (CPN2) domains each flanked by a 48 to 55 kDa catalytic (CPN1) domain. The C terminal region of the CPN1 subunit differs from all other family members in that it contains numerous basic residues. Proteolysis at these sites apparently happens either constitutively in the blood or during processing and secretion from the liver.