CPN1 Rekombinanter Antikörper

CPN1 Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

241481A5

Kat-Nr. : 84218-1-RR

Synonyme

Kininase 1, EC:3.4.17.3, CPN, Arginine carboxypeptidase, ACBP

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  WB analysis of human plasma using 84218-1-RR

  human plasma were subjected to SDS PAGE followed by western blot with 84218-1-RR (CPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 84218-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 84218-1-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 84218-1-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 84218-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84218-1-RR (CPN1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 84218-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CPN1 antibody (84218-1-RR, Clone: 241481A5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma
• Erfolgreiche Detektion in IHC: Mauslebergewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84218-1-RR bindet in WB, IHC, IF-P, ELISA CPN1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CPN1 fusion protein Ag4035
• Vollständiger Name: carboxypeptidase N, polypeptide 1
• Berechnetes Molekulargewicht: 458 aa, 52 kDa
• Beobachtetes Molekulargewicht: 40~50 kDa
• GenBank-Zugangsnummer: BC027897
• Gene symbol: CPN1
• Gene ID (NCBI): 1369
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. Normally, CPN circulates in blood plasma as a hetero-tetramer consisting of two 83 kDa (CPN2) domains each flanked by a 48 to 55 kDa catalytic (CPN1) domain. The C terminal region of the CPN1 subunit differs from all other family members in that it contains numerous basic residues. Proteolysis at these sites apparently happens either constitutively in the blood or during processing and secretion from the liver.