CPNE1 Polyklonaler Antikörper

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• KD/KO Validated

CPNE1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 10126-2-AP

Synonyme

Chromobindin 17, Copine 1, Copine I, Copine-1, copineI

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  WB analysis using 10126-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10126-2-AP (CPNE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 10126-2-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 10126-2-AP (CPNE1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human intrahepatic cholangiocarcinoma using 10126-2-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 10126-2-AP (CPNE1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 10126-2-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 10126-2-AP (CPNE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 10126-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10126-2-AP (CPNE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293T-Zellen
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue, Maus-Magengewebe, Ratten-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

10126-2-AP bindet in WB, IHC, ELISA CPNE1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPNE1 fusion protein Ag0139
• Vollständiger Name: copine I
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 59 kDa
• GenBank-Zugangsnummer: BC001142
• Gene symbol: CPNE1
• Gene ID (NCBI): 8904
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions.

Publikationen

Species	Application	Title
human	WB	Oncogene; Copine-III interacts with ErbB2 and promotes tumor cell migration.; Authors - C Heinrich
human	WB,IHC	Carcinogenesis; Copine 1 predicts poor clinical outcomes by promoting M2 macrophage activation in ovarian cancer; Authors - Bo Sheng
human	WB	MedComm (2020); Hsa-miR-335 enhances cell migration and invasion in lung adenocarcinoma through targeting Copine-1.; Authors - Yang Wang
human	WB	Front Biosci (Landmark Ed); Silencing of CPNE1-TRAF2 Axis Restrains the Development of Pancreatic Cancer; Authors - Yelin Song ; KO Validated
human	WB	Cell Death Discov; Circ_0000235 targets MCT4 to promote glycolysis and progression of bladder cancer by sponging miR-330-5p; Authors - Jianye Zhong

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