CPOX Polyklonaler Antikörper

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• KD/KO Validated

CPOX Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 12211-1-AP

Synonyme

Coprogen oxidase, Coproporphyrinogen oxidase, Coproporphyrinogenase, COX, CPO, CPOX, CPX, HCP

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Galerie der Validierungsdaten

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  WB analysis using 12211-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12211-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12211-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12211-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12211-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12211-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12211-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12211-1-AP (CPOX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 12211-1-AP

  IP Result of anti-CPOX (IP:12211-1-AP, 4ug; Detection:12211-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IHC staining of human liver cancer using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12211-1-AP (CPOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12211-1-AP (CPOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12211-1-AP (CPOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12211-1-AP (CPOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12211-1-AP (CPOX antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 12211-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12211-1-AP (CPOX antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

12211-1-AP bindet in WB, IP, IHC, IF, ELISA CPOX und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPOX fusion protein Ag2858
• Vollständiger Name: coproporphyrinogen oxidase
• Berechnetes Molekulargewicht: 454 aa, 50 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC023551
• Gene symbol: CPOX
• Gene ID (NCBI): 1371
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPOX(Coproporphyrinogen-III oxidase, mitochondrial) is also named as CPO and CPX. Human CPO is a 76 kDa protein that is active as a homodimer in the mitochondrial intermembrane pace(PMID:16159891).Mutations in human CPO gene predict the clinical outcome of the disease, with either hepatic hereditary coproporphyria or hematological manifestations of erythropoietic harderoporphyria.CPOX has a transit peptide of 110 aa and the chain contains 344 aa.

Publikationen

Species	Application	Title
human	WB, IF	J Cell Biol; Parkin-independent mitophagy via Drp1-mediated outer membrane severing and inner membrane ubiquitination.; Authors - Yumiko Oshima
human	IHC	Neuro Oncol; Enhanced expression of coproporphyrinogen oxidase in malignant brain tumors: CPOX expression and 5-ALA-induced fluorescence.; Authors - Takahashi Kenkichi K
human,mouse	WB	Biochem Pharmacol; Cystathionine-γ-lyase (CSE) deficiency increases erythropoiesis and promotes mitochondrial electron transport via the upregulation of coproporphyrinogen III oxidase and consequent stimulation of heme biosynthesis.; Authors - Katalin Módis ; KD Validated
human	IHC	Front Med (Lausanne); Heme Biosynthesis Factors and 5-ALA Induced Fluorescence: Analysis of mRNA and Protein Expression in Fluorescing and Non-fluorescing Gliomas.; Authors - Mario Mischkulnig
human	IHC	Photodiagnosis Photodyn Ther; Expression of coproporphyrinogen oxidase is associated with detection of upper gastrointestinal carcinomas by 5-aminolevulinic acid-mediated photodynamic diagnosis.; Authors - Kumi Ogihara
human	IHC	Photodiagnosis Photodyn Ther; Protoporphyrinogen oxidase is involved in the fluorescence intensity of 5-aminolevulinic acid-mediated laser-based photodynamic endoscopic diagnosis for early gastric cancer.; Authors - Hiroki Kurumi