CPSF1 Polyklonaler Antikörper

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• KD/KO Validated

CPSF1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11031-1-PBS

Synonyme

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  WB analysis of Jurkat using 11031-1-AP (same clone as 11031-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11031-1-AP (same clone as 11031-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 11031-1-AP (same clone as 11031-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 11031-1-AP (same clone as 11031-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11031-1-AP (CPSF1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11031-1-AP (same clone as 11031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11031-1-AP (same clone as 11031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 11031-1-AP (same clone as 11031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 11031-1-AP (same clone as 11031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 11031-1-AP (same clone as 11031-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11031-1-AP (CPSF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11031-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11031-1-PBS bindet in WB, IHC, Indirect ELISA CPSF1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPSF1 fusion protein Ag1489
• Vollständiger Name: cleavage and polyadenylation specific factor 1, 160kDa
• Berechnetes Molekulargewicht: 160 kDa
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: BC017232
• Gene symbol: CPSF1
• Gene ID (NCBI): 29894
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cleavage and polyadenylation specificity factor (CPSF) is a multisubunit complex that plays a central role in 3-prime processing of pre-mRNAs. CPSF recognizes the AAUAAA signal in the pre-mRNA and interacts with other proteins to facilitate both RNA cleavage and poly(A) synthesis.