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CPSF2 Rekombinanter Antikörper

CPSF2 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251387H5

Kat-Nr. : 86513-3-PBS

Synonyme

CPSF 100 kDa subunit, CPSF100, KIAA1367

Filter:
  • Western Blot (WB) analysis of various lysates using CPSF2 Recombinant antibody (86513-3-RR)

    WB analysis using 86513-3-RR (same clone as 86513-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86513-3-RR (CPSF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86513-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CPSF2 Recombinant antibody (86513-3-RR)

    IF Staining of HeLa using 86513-3-RR (same clone as 86513-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CPSF2 antibody (86513-3-RR, Clone: 251387H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86513-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86513-3-RR

    Affinity and Kinetic Characterization of 86513-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86513-3-RR against Human CPSF2 were performed. The affinity constant is 0.487 nM.

Geprüfte Anwendungen

Produktinformation

86513-3-PBS bindet in WB, IF/ICC, Indirect ELISA CPSF2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CPSF2 fusion protein Ag11950
Vollständiger Name cleavage and polyadenylation specific factor 2, 100kDa
Berechnetes Molekulargewicht 782 aa, 88 kDa
Beobachtetes Molekulargewicht 110 kDa
GenBank-ZugangsnummerBC070095
Gene symbol CPSF2
Gene ID (NCBI) 53981
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cleavage and Polyadenylation Specificity Factor Subunit 2 (CPSF2) is a critical component of the mRNA 3′ end processing machinery, playing a pivotal role in the cleavage and polyadenylation of pre-mRNA. This process is essential for the stability, nuclear export, and translation efficiency of mRNA, making CPSF2 a key player in gene expression regulation. Recent studies have highlighted the significance of CPSF2 in various diseases, particularly cancer. It's reported that in papillary thyroid carcinoma (PTC), negative expression of CPSF2 has been associated with poorer clinical outcomes, including higher rates of cervical lymph node metastasis, distant metastasis, and reduced recurrence-free survival. (PMID:26148673)