CPSF2 Rekombinanter Antikörper

CPSF2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

251387H5

Kat-Nr. : 86513-3-RR

Synonyme

CPSF 100 kDa subunit, CPSF100, KIAA1367

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  WB analysis using 86513-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86513-3-RR (CPSF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86513-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CPSF2 antibody (86513-3-RR, Clone: 251387H5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86513-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86513-3-RR against Human CPSF2 were performed. The affinity constant is 0.487 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, Maushirngewebe, Ramos-Zellen, RAW 264.7-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86513-3-RR bindet in WB, IF/ICC, ELISA CPSF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CPSF2 fusion protein Ag11950
• Vollständiger Name: cleavage and polyadenylation specific factor 2, 100kDa
• Berechnetes Molekulargewicht: 782 aa, 88 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC070095
• Gene symbol: CPSF2
• Gene ID (NCBI): 53981
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cleavage and Polyadenylation Specificity Factor Subunit 2 (CPSF2) is a critical component of the mRNA 3′ end processing machinery, playing a pivotal role in the cleavage and polyadenylation of pre-mRNA. This process is essential for the stability, nuclear export, and translation efficiency of mRNA, making CPSF2 a key player in gene expression regulation. Recent studies have highlighted the significance of CPSF2 in various diseases, particularly cancer. It's reported that in papillary thyroid carcinoma (PTC), negative expression of CPSF2 has been associated with poorer clinical outcomes, including higher rates of cervical lymph node metastasis, distant metastasis, and reduced recurrence-free survival. (PMID:26148673)