CPSF3 Polyklonaler Antikörper

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• KD/KO Validated

CPSF3 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 11609-1-AP

Synonyme

CPSF, CPSF 73, CPSF 73 kDa subunit, CPSF3, CPSF73, YSH1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11609-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11609-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skin using 11609-1-AP

  human skin tissue were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11609-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11609-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11609-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:700.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HepG2-Zellen, humanes Hautgewebe, humanes Hirngewebe
• Erfolgreiche IP: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below

Produktinformation

11609-1-AP bindet in WB, IP, ELISA CPSF3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPSF3 fusion protein Ag2173
• Vollständiger Name: cleavage and polyadenylation specific factor 3, 73kDa
• Berechnetes Molekulargewicht: 684 aa, 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC020211
• Gene symbol: CPSF3
• Gene ID (NCBI): 51692
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	J Exp Clin Cancer Res; LncRNA CASC9 interacts with CPSF3 to regulate TGF-β signaling in colorectal cancer.; Authors - Kaili Luo ; KD Validated
human	WB	Cancers (Basel); CPSF3 Promotes Pre-mRNA Splicing and Prevents CircRNA Cyclization in Hepatocellular Carcinoma; Authors - Ying Huang
human	WB	Cell Rep; Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.; Authors - J J David Ho
pig	WB	Cell Mol Life Sci; Single-cell multi-omics profiling reveals key regulatory mechanisms that poise germinal vesicle oocytes for maturation in pigs; Authors - Xiaolong Yuan
human	WB	Cell Chem Biol; Anticancer benzoxaboroles block pre-mRNA processing by directly inhibiting CPSF3; Authors - Ye Tao