CPSF3 Polyklonaler Antikörper

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CPSF3 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11609-1-PBS

Synonyme

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  WB analysis using 11609-1-AP (same clone as 11609-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 11609-1-AP (same clone as 11609-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 11609-1-AP (same clone as 11609-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 11609-1-AP (same clone as 11609-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 11609-1-AP (same clone as 11609-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11609-1-AP (CPSF3 antibody) at dilution of 1:700. This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 11609-1-AP (same clone as 11609-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 11609-1-AP (same clone as 11609-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 11609-1-AP (same clone as 11609-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 11609-1-AP (same clone as 11609-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11609-1-AP (CPSF3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11609-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11609-1-PBS bindet in WB, IHC, IP, Indirect ELISA CPSF3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CPSF3 fusion protein Ag2173
• Vollständiger Name: cleavage and polyadenylation specific factor 3, 73kDa
• Berechnetes Molekulargewicht: 684 aa, 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC020211
• Gene symbol: CPSF3
• Gene ID (NCBI): 51692
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPSF3 (cleavage and polyadenylation specificity factor 3) is involved in human disease and is the putative target of benzoxaboroles in trypanosomatid parasites and Cryptosporidium infection. CPSF3 has also been associated with cancer malignancy in that CPSF3 induces cell cycle arrest in hepatocellular carcinoma and promotes lung adenocarcinoma cell proliferation and metastasis (PMID: 38718887). CPSF3 has been implicated in various cancers, including lung cancer, colorectal, prostate, and triple-negative breast cancers (PMID: 37627085).