CPSF7 Polyklonaler Antikörper

CPSF7 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 55195-1-AP

Synonyme

CPSF 59 kDa subunit, CPSF7, FLJ12529

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  WB analysis using 55195-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 55195-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55195-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55195-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 55195-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55195-1-AP (CPSF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 55195-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55195-1-AP (CPSF7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 55195-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 55195-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 55195-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 55195-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 55195-1-AP (CPSF7 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 55195-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55195-1-AP (CPSF7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 55195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CPSF7 antibody (55195-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Hautgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

55195-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA CPSF7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: pre-mRNA cleavage factor I, 59 kDa subunit
• Berechnetes Molekulargewicht: 56 kDa
• Beobachtetes Molekulargewicht: 52 kDa, 56 kDa
• GenBank-Zugangsnummer: NM_001136040
• Gene symbol: CPSF7
• Gene ID (NCBI): 79869
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPSF7, also named as CPSF59, belongs to the RRM CPSF6/7 family. It probable component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. CPSF7 binds to cleavage and polyadenylation RNA substrates(PMID: 15489334). This antibody is specific to CPSF7.

Publikationen

Species	Application	Title
human	WB,IF	Cell Rep; CPSF6 regulates alternative polyadenylation and proliferation of cancer cells through phase separation; Authors - Susu Liu
human	CoIP	J Mol Cell Biol; U1 snRNP proteins promote proximal alternative polyadenylation sites by directly interacting with 3' end processing core factors; Authors - Zhijie Hu
		PLoS Pathog; Downregulation of CPSF6 Leads to Global mRNA 3' UTR shortening and enhanced antiviral immune responses; Authors - Yong Ge
human	WB	Mol Cell; 2'-O-methylation at internal sites on mRNA promotes mRNA stability; Authors - Yanqiang Li
human	WB	Cell Rep; E3 ligase SYVN1-mediated polyubiquitination of CPSF6 promotes alternative polyadenylation and antivirus effects of macrophages; Authors - Xin Lu
human	WB	Mol Cell; Mediator regulates transcriptional termination through crosstalk with pre-mRNA 3' end processing factors; Authors - Chengjiang Lu