CPT1A Monoklonaler Antikörper

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• KD/KO Validated

CPT1A Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5D8G9

Kat-Nr. : 66039-1-PBS

Synonyme

5D8G9, Carnitine O-palmitoyltransferase 1, liver isoform, Carnitine O-palmitoyltransferase I, liver isoform, CPT I, CPT1

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  WB analysis of A549 using 66039-1-Ig (same clone as 66039-1-PBS)

  WB result of CPT1A antibody (66039-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPT1A transfected A549 cells. This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 66039-1-Ig (same clone as 66039-1-PBS)

  IP result of anti-CPT1A (IP:66039-1-Ig, 4ug; Detection:66039-1-Ig 1:500) with MCF-7 cells lysate 1200ug. This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66039-1-Ig (same clone as 66039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66039-1-Ig (CPT1A antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66039-1-Ig (same clone as 66039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66039-1-Ig (CPT1A antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66039-1-Ig (same clone as 66039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66039-1-Ig(CPT1A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66039-1-Ig (same clone as 66039-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66039-1-Ig(CPT1A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66039-1-Ig (same clone as 66039-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CPT1A antibody (66039-1-Ig, Clone: 5D8G9 ) at dilution of 1:1800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66039-1-Ig (same clone as 66039-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug CPT1A Monoclonal antibody (66039-1-Ig, Clone:5D8G9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66039-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50197-1

  Cytometric bead array standard curve of MP50197-1, CPT1A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66039-1-PBS. Detection antibody: 66039-2-PBS. Standard:Ag7745. Range: 0.195-50 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66039-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Cytometric bead array, Indirect ELISA CPT1A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CPT1A fusion protein Ag7745
• Vollständiger Name: carnitine palmitoyltransferase 1A (liver)
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 86 kDa
• GenBank-Zugangsnummer: BC000185
• Gene symbol: CPT1A
• Gene ID (NCBI): 1374
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CPT1A, also named as CPT1, CPT1-L and L-CPTI, belongs to the carnitine/choline acetyltransferase family. Carnitine palmitoyltransferase (CPT) deficiencies are common disorders of mitochondrial fatty acid oxidation. The CPT system is made up of two separate proteins located in the outer (CPT1) and inner (CPT2) mitochondrial membranes. CPT1A is an active forms of related liver-type carnitine palmitoyltransferase I. (PMID: 11001805). CPT1A deficiency presents as recurrent attacks of fasting hypoketotic hypoglycemia. (PMID: 15363638). This antibody can bind the close sequences genes.