CPT2 Monoklonaler Antikörper

CPT2 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

3F11C10

Kat-Nr. : 68815-3-Ig

Synonyme

Carnitine O-palmitoyltransferase 2, mitochondrial, carnitine palmitoyltransferase 2, CPT II, CPTASE, EC:2.3.1.21

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  WB analysis of rat liver using 68815-3-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68815-3-Ig (CPT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68815-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68815-3-Ig (CPT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68815-3-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68815-3-Ig (CPT2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68815-3-Ig bindet in WB, ELISA CPT2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CPT2 fusion protein Ag24833
• Vollständiger Name: carnitine palmitoyltransferase 2
• Berechnetes Molekulargewicht: 74 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC002445
• Gene symbol: CPT2
• Gene ID (NCBI): 1376
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Carnitine palmitoyltransferase-1 and -2 (CPT1 and CPT2) are two genetically distinct mitochondrial membrane bound enzymes and play critical role in the regulation of FAO in normal cells. CPT2 is an ubiquitous protein and locates in the inner membrane of mitochondrial (PMID: 29437870). CPT2 is frequently down-regulated in primary ovarian serous carcinomas, which is significantly correlated with poor survival of ovarian cancer patients (PMID: 33486313). Down-regulation of CPT2 was a major cause of acylcarnitine accumulation and a common feature in mouse models of obesity- and NASH-driven HCC and human SH-HCC (PMID: 29872321).