CRABP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CRABP2 Polyklonal Antikörper für FC (Intra), IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC (Intra), CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

36

Rezensionen (3)

Kat-Nr. : 10225-1-AP

Synonyme

CRABP II, CRABP2, RBP6

---

Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 10225-1-AP

  WB result of CRABP2 antibody (10225-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRABP2 transfected HeLa cells.

• 

  WB analysis using 10225-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10225-1-AP (CRABP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10225-1-AP

  IP Result of anti-CRABP2 (IP:10225-1-AP, 3ug; Detection:10225-1-AP 1:300) with HeLa cells lysate 4650ug.

• 

  IHC staining of human skin using 10225-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10225-1-AP (CRABP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skin using 10225-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 10225-1-AP (CRABP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 10225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CRABP2 antibody (10225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of human skin cancer using 10225-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CRABP2 antibody (10225-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 10225-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human CRABP2 (10225-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HT-29-Zellen, Mausembryogewebe, MCF-7-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen, humanes Hautkrebsgewebe
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 6 publications below
• WB: See 23 publications below
• IHC: See 13 publications below
• IF: See 11 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Produktinformation

10225-1-AP bindet in WB, IP, IHC, IF, FC (Intra), CoIP, ELISA CRABP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRABP2 fusion protein Ag0309
• Vollständiger Name: cellular retinoic acid binding protein 2
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC001109
• Gene symbol: CRABP2
• Gene ID (NCBI): 1382
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

Publikationen

Species	Application	Title
mouse	IF	Science; A single progenitor population switches behavior to maintain and repair esophageal epithelium.; Authors - David P Doupé
human	WB	Mol Cell; TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.; Authors - Ai Kaiho-Soma
human	WB,IF	Neuron; Divergent Hox Coding and Evasion of Retinoid Signaling Specifies Motor Neurons Innervating Digit Muscles.; Authors - Alana I Mendelsohn
mouse	IF	Nat Commun; The developing mouse coronal suture at single-cell resolution.; Authors - D'Juan T Farmer
mouse	IF	Dev Cell; Single-Cell Transcriptomic Analyses of the Developing Meninges Reveal Meningeal Fibroblast Diversity and Function.; Authors - John DeSisto
human	IF	Stem Cell Reports; BLIMP1 Is Required for Postnatal Epidermal Homeostasis but Does Not Define a Sebaceous Gland Progenitor under Steady-State Conditions.; Authors - Kai Kretzschmar

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Yan (Verified Customer) (01-27-2020)

I used this antibody on mouse embryonic section for the immunofluorescence. It worked very well.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse embryonic section

FH

lunfeng (Verified Customer) (01-27-2020)

VERY GOOD

• Applications: Immunofluorescence,

FH

Jie (Verified Customer) (01-27-2020)

VERY GOOD ANTIBODY. WORKS IN KO VERSUS WT CONTROL MICE.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: mouse brain tissue

Verwandte Produkte

CRABP1 Polyclonal antibody

12588-1-AP

FABP5 Polyclonal antibody

12348-1-AP

RXRA Monoclonal antibody

60198-1-Ig

PPARA Polyclonal antibody

15540-1-AP

RBP1 Polyclonal antibody

22683-1-AP