CRABP2 Polyklonaler Antikörper

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• KD/KO Validated

CRABP2 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10225-1-PBS

Synonyme

Cellular retinoic acid binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid-binding protein II, CRABP II, CRABP-II

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  WB analysis of HeLa using 10225-1-AP (same clone as 10225-1-PBS)

  WB result of CRABP2 antibody (10225-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRABP2 transfected HeLa cells. This data was developed using the same antibody clone with 10225-1-PBS in a different storage buffer formulation.

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  WB analysis using 10225-1-AP (same clone as 10225-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10225-1-AP (CRABP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10225-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 10225-1-AP (same clone as 10225-1-PBS)

  IP result of anti-CRABP2 (IP:10225-1-AP, 3ug; Detection:10225-1-AP 1:300) with HeLa cells lysate 4650ug. This data was developed using the same antibody clone with 10225-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 10225-1-AP (same clone as 10225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 10225-1-AP (CRABP2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10225-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse embryo using 10225-1-AP (same clone as 10225-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 10225-1-AP (CRABP2 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10225-1-PBS in a different storage buffer formulation.

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  IF Staining of human skin cancer using 10225-1-AP (same clone as 10225-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CRABP2 antibody (10225-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10225-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 10225-1-AP (same clone as 10225-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CRABP2 antibody (10225-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10225-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 10225-1-AP (same clone as 10225-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human CRABP2 (10225-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10225-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10225-1-PBS bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA CRABP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRABP2 fusion protein Ag0309
• Vollständiger Name: cellular retinoic acid binding protein 2
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC001109
• Gene symbol: CRABP2
• Gene ID (NCBI): 1382
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

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