CRABP2 Monoklonaler Antikörper

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CRABP2 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

1A5F3

Kat-Nr. : 66468-1-PBS

Synonyme

1A5F3, Cellular retinoic acid binding protein II, Cellular retinoic acid-binding protein 2, Cellular retinoic acid-binding protein II, CRABP II

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  WB analysis of T-47D using 66468-1-Ig (same clone as 66468-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 66468-1-Ig (CRABP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66468-1-Ig (same clone as 66468-1-PBS)

  WB result of CRABP2 antibody (66468-1-Ig; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRABP2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66468-1-Ig (same clone as 66468-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66468-1-Ig (CRABP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

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  WB analysis of rat skin using 66468-1-Ig (same clone as 66468-1-PBS)

  rat skin tissue were subjected to SDS PAGE followed by western blot with 66468-1-Ig (CRABP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

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  WB analysis of pig skin using 66468-1-Ig (same clone as 66468-1-PBS)

  pig skin tissue were subjected to SDS PAGE followed by western blot with 66468-1-Ig (CRABP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66468-1-Ig (same clone as 66468-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66468-1-Ig (CRABP2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66468-1-Ig (same clone as 66468-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66468-1-Ig (CRABP2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

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  IF Staining of human skin cancer using 66468-1-Ig (same clone as 66468-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CRABP2 antibody (66468-1-Ig, Clone: 1A5F3 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66468-1-Ig (same clone as 66468-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CRABP2 antibody (66468-1-Ig, Clone: 1A5F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 66468-1-Ig (same clone as 66468-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human CRABP2 (66468-1-Ig, Clone:1A5F3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66468-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66468-1-PBS bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA CRABP2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CRABP2 fusion protein Ag0309
• Vollständiger Name: cellular retinoic acid binding protein 2
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC001109
• Gene symbol: CRABP2
• Gene ID (NCBI): 1382
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.