CRABP2 Monoklonaler Antikörper

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• KD/KO Validated

CRABP2 Monoklonal Antikörper für FC (Intra), IF

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1A5F3

Kat-Nr. : CL594-66468

Synonyme

CRABP II, CRABP2, RBP6

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Galerie der Validierungsdaten

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  IF Staining of human skin cancer using CL594-66468

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using CoraLite®594-conjugated CRABP2 antibody (CL594-66468, Clone: 1A5F3 ) at dilution of 1:100.

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  FC experiment of MCF-7 using CL594-66468

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human CRABP2 (CL594-66468, Clone:1A5F3) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: humanes Hautkrebsgewebe
• Erfolgreiche Detektion in FC: MCF-7-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66468 bindet in IF, FC (Intra) CRABP2 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CRABP2 fusion protein Ag0309
• Vollständiger Name: cellular retinoic acid binding protein 2
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC001109
• Gene symbol: CRABP2
• Gene ID (NCBI): 1382
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cellular retinoic acid binding protein 2 (CRABP2, synonyms: RBP6, CRABP-II). A number of specific carrier proteins for members of the vitamin A family have been discovered. Cellular retinoic acid binding proteins (CRABP) are low molecular weight proteins whose precise function remains unknown. CRABP2 is important in retinoic acid-mediated regulation of human skin growth and differentiation. It has been postulated that the CRABP2 gene is transcriptionally regulated by a newly synthesized regulatory protein.

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PF00011