CRACR2A Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CRACR2A Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4C1F5

Kat-Nr. : 66787-1-PBS

Synonyme

EFCAB4B, EF-hand calcium-binding domain-containing protein 4B, CRAC channel regulator 2A, Calcium release-activated channel regulator 2A, Calcium release-activated calcium channel regulator 2A

---

• 

  WB analysis using 66787-1-Ig (same clone as 66787-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66787-1-Ig (CRACR2A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66787-1-Ig (same clone as 66787-1-PBS)

  WB result of CRACR2A antibody (66787-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CRACR2A transfected Jurkat cells. This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66787-1-Ig (same clone as 66787-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66787-1-Ig (CRACR2A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 66787-1-Ig (same clone as 66787-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 66787-1-Ig (CRACR2A antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66787-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66787-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CRACR2A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CRACR2A fusion protein Ag7525
• Vollständiger Name: EF-hand calcium binding domain 4B
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 80-90 kDa
• GenBank-Zugangsnummer: BC004524
• Gene symbol: CRACR2A
• Gene ID (NCBI): 84766
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRACR2A (also called CRAC regulator 2A, EFCAB4B) is a cytoplasmic Ca2+ sensor predominantly expressed in lymphocytes. It has two isoforms: a long isoform of CRACR2A-a round 80-90 kDa and a short isoform of CRACR2A-c around 46 kDa. CRACR2A-c is involved in the regulation of Ca2+-NFAT signaling. CRACR2A-a exists in a GTP- and Golgi membrane-bound form in resting T cells, while it is present in subsynaptic vesicles that translocated from the Golgi region to the immunological synapse after ligation of TCRs. This antibody recognizes both of CRACR2A-c and CRACR2A-a.