CRB1 Polyklonaler Antikörper

CRB1 Polyklonal Antikörper für IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26598-1-PBS

Synonyme

Crumbs homolog 1, crumbs homolog 1 (Drosophila), LCA8, Protein crumbs homolog 1, RP12

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  IHC staining of mouse eye using 26598-1-AP (same clone as 26598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 26598-1-AP (same clone as 26598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 26598-1-AP (same clone as 26598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 26598-1-AP (same clone as 26598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 26598-1-AP (same clone as 26598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 26598-1-AP (same clone as 26598-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 26598-1-AP (same clone as 26598-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using CRB1 antibody (26598-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 26598-1-AP (same clone as 26598-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CRB1 antibody (26598-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26598-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

26598-1-PBS bindet in IHC, IF/ICC, IF-P, Indirect ELISA CRB1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRB1 fusion protein Ag24512
• Vollständiger Name: crumbs homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 1406 aa, 154 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC136271
• Gene symbol: CRB1
• Gene ID (NCBI): 23418
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRB1, also known as protein crumbs homolog 1, is homologous to Drosophila Crumbs, a protein that is essential for establishing and maintaining apico-basal polarity in epithelia derived from the ectoderm (PMID: 2344615). CRB1 protein is a single transmembrane protein with a large extracellular part, containing three laminin A G domains and 19 epidermal growth-factor-like domains (PMID: 15494026, 18407265, 11734541). The expression of CRB1 is restricted to the retina and brain (PMID: 10508521). Mutations in CRB1 gene cause severe retinal dystrophies, ranging from retinitis pigmentosa (RP) to Leber congenital amaurosis (LCA) (PMID: 11734541).