CRCP Polyklonaler Antikörper

CRCP Polyklonal Antikörper für FC, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 14348-1-AP

Synonyme

CGRP RCP, CGRP receptor component, CGRPRCP, CRCP, HsC17, RCP, RCP9, RNA polymerase III subunit C9

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Galerie der Validierungsdaten

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  WB analysis of Raji using 14348-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 14348-1-AP (CRCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14348-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14348-1-AP (CRCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14348-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14348-1-AP (CRCP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 14348-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14348-1-AP (CRCP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 14348-1-AP

  IP Result of anti-CRCP (IP:14348-1-AP, 3ug; Detection:14348-1-AP 1:1000) with Raji cells lysate 2800ug.

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  IHC staining of human testis using 14348-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14348-1-AP (CRCP Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 14348-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14348-1-AP (CRCP Antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of Raji using 14348-1-AP

  1X10^6 Raji cells were stained with 0.2ug CRCP antibody (14348-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HeLa-Zellen, humanes Hodengewebe, MCF-7-Zellen
• Erfolgreiche IP: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Raji-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 5 publications below

Produktinformation

14348-1-AP bindet in WB, IP, IHC, IF, FC, ELISA CRCP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CRCP fusion protein Ag5689
• Vollständiger Name: CGRP receptor component
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC040107
• Gene symbol: CRCP
• Gene ID (NCBI): 27297
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse,rat	IF	J Neuroinflammation; Calcitonin gene-related peptide regulates spinal microglial activation through the histone H3 lysine 27 trimethylation via enhancer of zeste homolog-2 in rats with neuropathic pain.; Authors - Qi An
human	WB	J Transl Med; CircESRP1 enhances metastasis and epithelial-mesenchymal transition in endometrial cancer via the miR-874-3p/CPEB4 axis.; Authors - Rui Shi
rat	IF,WB	CNS Neurosci Ther; Calcitonin gene-related peptide induces the histone H3 lysine 9 acetylation in astrocytes associated with neuroinflammation in rats with neuropathic pain.; Authors - Chenyan Sun
mouse	IF	Neurogastroenterol Motil; Altered gene expression signatures by calcitonin gene-related peptide promoted mast cell activity in the colon of stress-induced visceral hyperalgesia mice.; Authors - Hanliang Sun
rat	IF	Cell Biochem Funct; CNTs-CaP/chitosan-coated AZ91D magnesium alloy extract promoted rat dorsal root ganglia neuron growth via activating ERK signalling pathway.; Authors - Tingjiao Liu
mouse	IF	J Immunol Res; ChIP-seq Profiling Identifies Histone Deacetylase 2 Targeting Genes Involved in Immune and Inflammatory Regulation Induced by Calcitonin Gene-Related Peptide in Microglial Cells.; Authors - Xingjing Guo

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