CREB3 Monoklonaler Antikörper

CREB3 Monoklonal Antikörper für WB, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C12H11

Kat-Nr. : 68379-1-PBS

Synonyme

Leucine zipper protein, Cyclic AMP-responsive element-binding protein 3, CREB-3, CREB 3, cAMP-responsive element-binding protein 3

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  WB analysis of T-47D using 68379-1-Ig (same clone as 68379-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68379-1-PBS in a different storage buffer formulation.

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  WB analysis of HCT 116 using 68379-1-Ig (same clone as 68379-1-PBS)

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68379-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68379-1-Ig (same clone as 68379-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68379-1-Ig (CREB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68379-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 68379-1-Ig (same clone as 68379-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CREB3 antibody (68379-1-Ig, Clone: 1C12H11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68379-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50828-4

  Cytometric bead array standard curve of MP50828-4, CREB3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68379-7-PBS. Detection antibody: 68379-1-PBS. Standard:Ag1823. Range: 1.563-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

68379-1-PBS bindet in WB, IF/ICC, Cytometric bead array, Indirect ELISA CREB3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CREB3 fusion protein Ag1823
• Vollständiger Name: cAMP responsive element binding protein 3
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 40-64 kDa
• GenBank-Zugangsnummer: BC009402
• Gene symbol: CREB3
• Gene ID (NCBI): 10488
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CREB3, also named as LZIP and Luman, belongs to the bZIP family and ATF subfamily. It is a transcription factors activated upon intramembrane proteolysis (RIP), binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. CREB3 binds to and requires HCFC1 as a coactivator. The activity and expression are suppressed when the HCFC1-CREB3 complex binds with CREBZF. Participates in LKN-1/CCL15-induced chemotaxis signaling. CREB3 is N-glycosylated and released by proteolysis. So the MW of CREB3 emirate 40kd to 64kd (PMID: 12138176).