CREB3L1,OASIS Polyklonaler Antikörper

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• KD/KO Validated

CREB3L1,OASIS Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11235-2-PBS

Synonyme

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  WB analysis of A549 using 11235-2-AP (same clone as 11235-2-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This batch of A549 cells lysate predominantly expressed the maturer form likely due to cell stage. This data was developed using the same antibody clone with 11235-2-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 11235-2-AP (same clone as 11235-2-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11235-2-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 11235-2-AP (same clone as 11235-2-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This batch of A549 cells lysate predominantly expressed the precursor form likely due to cell stage. This data was developed using the same antibody clone with 11235-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 11235-2-AP (same clone as 11235-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:50 (under 25x lens). This data was developed using the same antibody clone with 11235-2-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 11235-2-AP (same clone as 11235-2-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 11235-2-AP (CREB3L1,OASIS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11235-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11235-2-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CREB3L1,OASIS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CREB3L1,OASIS fusion protein Ag1757
• Vollständiger Name: cAMP responsive element binding protein 3-like 1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 55-58 kDa and 84 kDa
• GenBank-Zugangsnummer: BC015781
• Gene symbol: CREB3L1/OASIS
• Gene ID (NCBI): 90993
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cyclic AMP-responsive element-binding protein 3-like protein 1(CREB3L1) belongs to a family of transcription factors that are synthesized as membrane-bound precursors in the endoplasmic reticulum (ER) and then transported to the Golgi where they are activated through regulated intramembrane proteolysis (RIP). Also it participates in ER-stress response in astrocytes in the central nervous system. It may has a role in gliosis, and involves in activation of transcription through box-B element but not CRE. CREB3L1 acts as a transcription repressor in proliferation of virus-infected cell. The full-length precursor form (approximately 84 kDa) of CREB3L1 and the matured form of CREB3L1 is 58 kDa. (PMID: 20668028)